Abstract
Steroid receptor RNA activator (SRA) was first isolated as a steroid receptor co-activator that functioned as an RNA transcript. Later, we demonstrated that SRA needs to be translated in order to co-activate androgen receptor (AR). Here, we showed that three isoforms of human SRA enhanced AR activities. Small interfering RNA against SRA suppressed AR activities in PC-3 cells transfected with pSG5AR and in LNCaP cells that have an endogenous mutated-AR. Western blot showed that SRA protein was expressed at a higher level in PC-3 than in LNCaP cells, suggesting that SRA may be related to hormone-independent growth of prostate cancer.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Biomarkers, Tumor / analysis
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Blotting, Western
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Disease Progression
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Gene Expression Regulation, Neoplastic*
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Humans
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Male
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Molecular Sequence Data
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Prostatic Neoplasms / genetics*
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Prostatic Neoplasms / pathology
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RNA, Long Noncoding
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RNA, Neoplasm / analysis
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RNA, Untranslated / genetics*
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RNA, Untranslated / metabolism
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Receptors, Androgen / genetics
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Receptors, Androgen / metabolism
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Receptors, Androgen / physiology*
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Reverse Transcriptase Polymerase Chain Reaction
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Risk Factors
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Sampling Studies
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Sensitivity and Specificity
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Tumor Cells, Cultured / cytology
Substances
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AR protein, human
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Biomarkers, Tumor
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RNA, Long Noncoding
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RNA, Neoplasm
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RNA, Untranslated
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Receptors, Androgen
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steroid receptor RNA activator