Expression and function of human steroid receptor RNA activator in prostate cancer cells: role of endogenous hSRA protein in androgen receptor-mediated transcription

Prostate Cancer Prostatic Dis. 2006;9(2):173-8. doi: 10.1038/sj.pcan.4500867. Epub 2006 Apr 11.


Steroid receptor RNA activator (SRA) was first isolated as a steroid receptor co-activator that functioned as an RNA transcript. Later, we demonstrated that SRA needs to be translated in order to co-activate androgen receptor (AR). Here, we showed that three isoforms of human SRA enhanced AR activities. Small interfering RNA against SRA suppressed AR activities in PC-3 cells transfected with pSG5AR and in LNCaP cells that have an endogenous mutated-AR. Western blot showed that SRA protein was expressed at a higher level in PC-3 than in LNCaP cells, suggesting that SRA may be related to hormone-independent growth of prostate cancer.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Biomarkers, Tumor / analysis
  • Blotting, Western
  • Disease Progression
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Male
  • Molecular Sequence Data
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / pathology
  • RNA, Long Noncoding
  • RNA, Neoplasm / analysis
  • RNA, Untranslated / genetics*
  • RNA, Untranslated / metabolism
  • Receptors, Androgen / genetics
  • Receptors, Androgen / metabolism
  • Receptors, Androgen / physiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Risk Factors
  • Sampling Studies
  • Sensitivity and Specificity
  • Tumor Cells, Cultured / cytology


  • AR protein, human
  • Biomarkers, Tumor
  • RNA, Long Noncoding
  • RNA, Neoplasm
  • RNA, Untranslated
  • Receptors, Androgen
  • steroid receptor RNA activator