[Screening methods for detection of metallo-beta-lactamase producing gram negative rods]

Rev Med Chir Soc Med Nat Iasi. Apr-Jun 2005;109(2):387-91.
[Article in Romanian]

Abstract

Modified Hodge test and a method using a disk with imipenem plus 1000 mg of EDTA were used to determine the presence of metallo-beta-lactamase producing gram-negative rods among 166 clinical isolates from hospitals in Iaşi and Galaţi. Of 9 imipenem resistant strains found, only one Pseudomonas aeruginosa gave positive results with both tests and other two P. aeruginosa clinical isolates gave negative results with both tests. The rest of the strains (2 P. aeruginosa, 2 Acinetobacter baumanii, 1 Sphingomonas paucimobilis) did not give conclusive results. These screening methods are useful, simple and accessible to clinical laboratories. PCR is needed to confirm the presence of metallo-beta-lactamase gene in bacteria and to determine the type of the enzymes.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / analysis*
  • Bacterial Proteins / biosynthesis
  • Blood / microbiology
  • Carbapenems / pharmacology
  • Cerebrospinal Fluid / microbiology
  • Drug Resistance, Bacterial
  • Gram-Negative Bacteria / drug effects
  • Gram-Negative Bacteria / enzymology*
  • Humans
  • Mass Screening
  • Microbial Sensitivity Tests
  • Multicenter Studies as Topic
  • Pleural Effusion / microbiology
  • Polymerase Chain Reaction
  • Pseudomonas aeruginosa / drug effects
  • Pseudomonas aeruginosa / enzymology*
  • Romania
  • Suppuration / microbiology
  • Urine / microbiology
  • beta-Lactamases / analysis*
  • beta-Lactamases / biosynthesis

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carbapenems
  • beta-Lactamases
  • cphA protein, Aeromonas hydrophila