Anti type I allergic property of Japanese butterbur extract and its mast cell degranulation inhibitory ingredients

J Agric Food Chem. 2006 Apr 19;54(8):2915-20. doi: 10.1021/jf052994o.


Pollenosis is a disease that affects 1 in 10 of the Japanese population. During the season of cedar pollen dispersal, many patients suffer from symptoms such as sniffling, sternutation, and itching of the eyes. Japanese butterbur is a popular vegetable and is one of the few domestic vegetables in Japan. The anti type I allergic effects of an aqueous ethanol extract from aerial parts of Japanese butterbur (JBE) were evaluated in rats and RBL-2H3 mast cells. In the passive cutaneous anaphylaxis reaction in rats, a single oral treatment of JBE (1000 mg/kg) was found to suppress the reaction. In IgE-sensitized RBL-2H3 cells, JBE (10-100 microg/mL) inhibited beta-hexosaminidase release, leukotriene C(4)/D(4)/E(4) synthesis, and TNF-alpha production. Moreover, a high concentration of JBE (1000 microg/mL) suppressed smooth muscle constriction induced by histamine (10 microM) and leukotriene D(4) (10 nM) in a guinea pig trachea strip. The search for components in JBE with an inhibitory activity on mast cell degranulation was guided by inhibition of beta-hexsosaminidase release. Two eremophilane-type sesquiterpenes, six polyphenolic compounds, and two triterpene glycosides were isolated. Of these compounds, fukinolic acid, a principal polyphenol constituent, showed potent inhibitory activity (IC(50) value = 2.1 microg/mL). Consequently, On the basis of its inhibition of mast cell activation and direct smooth muscle reaction induced by released mediators, JBE was found to suppress the type I allergic reaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Degranulation / drug effects*
  • Cell Line
  • Female
  • Guinea Pigs
  • Histamine / pharmacology
  • Leukotriene D4 / pharmacology
  • Male
  • Mast Cells / drug effects*
  • Mast Cells / immunology
  • Muscle Contraction / drug effects
  • Muscle, Smooth / physiopathology
  • Petasites / chemistry*
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Rats
  • Rats, Wistar
  • Rhinitis, Allergic, Seasonal / physiopathology
  • Rhinitis, Allergic, Seasonal / prevention & control*
  • Trachea / physiopathology


  • Plant Extracts
  • Leukotriene D4
  • Histamine