How to stabilize phospholipid liposomes (using nanoparticles)

Nano Lett. 2006 Apr;6(4):694-8. doi: 10.1021/nl052455y.


The simple strategy of mixing phospholipid liposomes with charged nanoparticles and using sonication to mix them at low volume fraction produces particle-stabilized liposomes that repel one another and do not fuse. Subsequently, the volume fraction can be raised as high as approximately 50%, reversibly, still without fusion. In studies of liposome longevity, we verified the stability of particle-stabilized liposome suspensions with volume fraction up to 16% for up to 50 days, the longest period investigated. Fluorescent dyes were encapsulated within the particle-stabilized liposomes, without leakage. Although these particle-stabilized liposomes were stable against fusion, approximately 75% of the outer liposome surface remained unoccupied. This opens the door to using particle-stabilized liposomes in various applications.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Lipid Bilayers / analysis
  • Lipid Bilayers / chemistry*
  • Liposomes / analysis
  • Liposomes / chemistry*
  • Materials Testing
  • Membrane Fluidity*
  • Membrane Fusion*
  • Molecular Conformation
  • Nanostructures / chemistry*
  • Nanostructures / ultrastructure*
  • Particle Size
  • Phospholipids / analysis
  • Phospholipids / chemistry*


  • Lipid Bilayers
  • Liposomes
  • Phospholipids