Tissue distribution of the 1,25-dihydroxyvitamin D3 receptor in the male rat

Biochem Biophys Res Commun. 1991 Dec 16;181(2):611-6. doi: 10.1016/0006-291x(91)91234-4.


Tissue distribution of 1,25-dihydroxyvitamin D3 receptors was studied in male rats using a quantitative immunoradiometric assay. Extracts were prepared from 16 different rat tissues and assayed for 1,25-dihydroxyvitamin D3 receptor. Measurable levels of receptor were detected in intestine, stomach, kidney, bone thyroid/parathyroid, skin, liver, spleen, heart and lung. The highest levels were found in the proximal small intestine and colon, containing over 1000 fmol/mg total protein, while ileum and kidney contained one-half and one-fourth of this amount, respectively. Other parts of the vitamin D endocrine system, including bone, thyroid/parathyroid and skin, contained moderate levels of receptor of 40 to 80 fmol/mg, while lung, heart, stomach, spleen and liver had levels at or below 20 fmol/mg. No 1,25-dihydroxyvitamin D3 receptor was detected in cerebrum, cerebellum or skeletal muscle. The data support a wide-spread role for 1,25-dihydroxyvitamin D3 on cellular processes and suggest a more important role for vitamin D in colon.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Colon / chemistry
  • Immunoradiometric Assay
  • Intestine, Small / chemistry
  • Kidney / chemistry
  • Male
  • Rats
  • Rats, Inbred Strains
  • Receptors, Calcitriol
  • Receptors, Steroid / analysis*
  • Skin / chemistry
  • Tissue Distribution


  • Receptors, Calcitriol
  • Receptors, Steroid