Two-dimensional electrophoretic proteome study of serum thermostable fraction from patients with various tumor conditions

Biochemistry (Mosc). 2006 Apr;71(4):354-60. doi: 10.1134/s000629790604002x.


One of the problems of plasma proteomics is a presence of large major components. In this work, we use the thermostable fraction as a way to deplete these major proteins. The thermostable fraction of serum samples from patients with ovarian, uterus, and breast cancers and benign ovarian tumor was analyzed using two-dimensional electrophoresis combined with MALDI-TOF(-TOF)-mass spectrometry. Of them, alpha-1-acid glycoprotein and clusterin are expressly down-regulated in breast cancer, whereas transthyretin is decreased specifically in ovarian cancer. Apolipoprotein A-I forms have decreased spot volumes, while haptoglobin alpha1, in contrast, is elevated in several tumors. These data are partly consistent with previous art studies on cancer proteomics, which involve mass-spectrometry-based serum profiling techniques. Serum thermostable fraction may be recommended as a good tool for medium and small protein proteome investigation, in particular, by 2D-electrophoresis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Biomarkers, Tumor / blood*
  • Breast Neoplasms / blood
  • Breast Neoplasms / metabolism
  • Electrophoresis, Gel, Two-Dimensional / methods*
  • Female
  • Humans
  • Middle Aged
  • Neoplasm Proteins / blood*
  • Ovarian Neoplasms / blood
  • Ovarian Neoplasms / metabolism
  • Proteome / analysis*
  • Proteomics / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Uterine Neoplasms / blood
  • Uterine Neoplasms / metabolism


  • Biomarkers, Tumor
  • Neoplasm Proteins
  • Proteome