AlOH3-adjuvanted Vaccine-Induced Macrophagic Myofasciitis in Rats Is Influenced by the Genetic Background

Neuromuscul Disord. 2006 May;16(5):347-52. doi: 10.1016/j.nmd.2006.02.004. Epub 2006 Apr 17.

Abstract

Macrophagic myofasciitis (MMF) is a specific histopathologic lesion involved in the persistence for years of aluminum hydroxide [Al(OH)(3)] at the site of previous intramuscular (i.m.) injection. In order to study mechanisms involved persistence of MMF lesions, we set up an experimental model of MMF-lesion in Sprague-Dawley and Lewis rat, by i.m. injections of 10 microL of an Al(OH)(3)-adjuvanted vaccine. An evaluation carried out over a 12-month period disclosed significant shrinkage of MMF lesions with time. A radioisotopic study did not show significant aluminium uptake by Al(OH)(3)-loaded macrophages. A morphometric approach showed that Lewis rats with Th1-biased immunity had significantly smaller lesions than Sprague-Dawley rats with balanced Th1/Th2 immunity. Concluding, our results indicate that genetic determinatives of cytotoxic T-cell responses could interfere with the clearance process and condition the persistence of vaccine-induced MMF-lesions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aluminum Hydroxide / adverse effects*
  • Animals
  • Disease Models, Animal
  • Female
  • Genetic Predisposition to Disease / genetics*
  • Immunity, Cellular / drug effects
  • Immunity, Cellular / immunology
  • Injections, Intramuscular / adverse effects
  • Macrophages / drug effects*
  • Macrophages / immunology
  • Muscle, Skeletal / drug effects
  • Muscle, Skeletal / immunology
  • Muscle, Skeletal / pathology
  • Myofascial Pain Syndromes / chemically induced*
  • Myofascial Pain Syndromes / genetics*
  • Myofascial Pain Syndromes / physiopathology
  • Rats
  • Rats, Inbred Lew
  • Rats, Sprague-Dawley
  • T-Lymphocytes, Cytotoxic / drug effects
  • T-Lymphocytes, Cytotoxic / immunology
  • Th1 Cells / drug effects
  • Th1 Cells / immunology
  • Th2 Cells / drug effects
  • Th2 Cells / immunology
  • Vaccines / adverse effects*

Substances

  • Vaccines
  • Aluminum Hydroxide