[Purification of a new phospholipase A2 homologue from Agkistrodon blomhoffii siniticus and its effects on gene expression of Hep3B cells]

Zhong Yao Cai. 2006 Feb;29(2):132-4.
[Article in Chinese]

Abstract

A new PLA2 homologue was purified from Agkistrodon blomhoffii Siniticus by applying reverse phase (HPLC) C18 column. The molecular weight of PLA2 homologue is 13900Da and its purity is 97.2%. The results of N-terminal sequence analysis were HLLQFRKMIKKMTKK. All the fragments were determined with the protein-protein BLAST software of GenBank. BLAST analysis showed that the N-terminal sequences of 15 amino acids were highly homologous with the sequences of other PLA2.

MeSH terms

  • Agkistrodon*
  • Amino Acid Sequence
  • Animals
  • Chromatography, High Pressure Liquid / methods
  • Molecular Sequence Data
  • Molecular Weight
  • Phospholipases A / chemistry
  • Phospholipases A / isolation & purification*
  • Phospholipases A2
  • Sequence Analysis, Protein
  • Sequence Homology, Amino Acid
  • Snake Venoms / chemistry*

Substances

  • Snake Venoms
  • Phospholipases A
  • Phospholipases A2