Construction of cloning vectors for Bacillus thuringiensis

Gene. 1991 Dec 1;108(1):115-9. doi: 10.1016/0378-1119(91)90495-w.

Abstract

The replication region of the Bacillus thuringiensis plasmid, pHT1030, was treated with hydroxylamine. Various copy-number mutants were selected and subsequently used to construct shuttle vectors with multiple cloning sites. These recombinant plasmids are very stable and allowed the cloning of a delta-endotoxin-encoding gene in B. thuringiensis. Comparison between gene expression level and vector copy-number indicated that a plateau in delta-endotoxin production is reached with a copy-number of about fifteen per equivalent chromosome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus thuringiensis / genetics*
  • Bacterial Proteins*
  • Bacterial Toxins*
  • Cloning, Molecular
  • DNA Replication / genetics*
  • Endotoxins / genetics
  • Gene Expression / genetics
  • Genetic Vectors / genetics*
  • Hemolysin Proteins
  • Hydroxylamine
  • Hydroxylamines
  • Mutagenesis
  • Plasmids / genetics*
  • Recombination, Genetic / genetics

Substances

  • Bacterial Proteins
  • Bacterial Toxins
  • Endotoxins
  • Hemolysin Proteins
  • Hydroxylamines
  • insecticidal crystal protein, Bacillus Thuringiensis
  • Hydroxylamine