Identification of SNAP-47, a novel Qbc-SNARE with ubiquitous expression

J Biol Chem. 2006 Jun 23;281(25):17076-17083. doi: 10.1074/jbc.M513838200. Epub 2006 Apr 18.


The SNARE proteins are essential components of the intracellular fusion machinery. It is thought that they form a tight four-helix complex between membranes, in effect initiating fusion. Most SNAREs contain a single coiled-coil region, referred to as the SNARE motif, directly adjacent to a single transmembrane domain. The neuronal SNARE SNAP-25 defines a subfamily of SNARE proteins with two SNARE helices connected by a longer linker, comprising also the proteins SNAP-23 and SNAP-29. We now report the initial characterization of a novel vertebrate homologue termed SNAP-47. Northern blot and immunoblot analysis revealed ubiquitous tissue distribution, with particularly high levels in nervous tissue. In neurons, SNAP-47 shows a widespread distribution on intracellular membranes and is also enriched in synaptic vesicle fractions. In vitro, SNAP-47 substituted for SNAP-25 in SNARE complex formation with the neuronal SNAREs syntaxin 1a and synaptobrevin 2, and it also substituted for SNAP-25 in proteoliposome fusion. However, neither complex assembly nor fusion was as efficient as with SNAP-25.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Gene Expression Regulation*
  • Humans
  • Molecular Sequence Data
  • Neurons / metabolism
  • Qb-SNARE Proteins / biosynthesis*
  • Qb-SNARE Proteins / chemistry
  • Qc-SNARE Proteins / biosynthesis*
  • Qc-SNARE Proteins / chemistry
  • Rats
  • SNARE Proteins / biosynthesis
  • SNARE Proteins / physiology*
  • Synaptosomal-Associated Protein 25 / biosynthesis
  • Tissue Distribution
  • Vesicular Transport Proteins / biosynthesis


  • Qb-SNARE Proteins
  • Qc-SNARE Proteins
  • SNARE Proteins
  • Snap23 protein, mouse
  • Snap23 protein, rat
  • Snap29 protein, mouse
  • Snap29 protein, rat
  • Snap47 protein, mouse
  • Snap47 protein, rat
  • Synaptosomal-Associated Protein 25
  • Vesicular Transport Proteins