Comparative Proteomics of Glycosomes From Bloodstream Form and Procyclic Culture Form Trypanosoma Brucei Brucei

Proteomics. 2006 Jun;6(11):3275-93. doi: 10.1002/pmic.200500668.

Abstract

Peroxisomes are present in nearly every eukaryotic cell and compartmentalize a wide range of important metabolic processes. Glycosomes of Kinetoplastid parasites are peroxisome-like organelles, characterized by the presence of the glycolytic pathway. The two replicating stages of Trypanosoma brucei brucei, the mammalian bloodstream form (BSF) and the insect (procyclic) form (PCF), undergo considerable adaptations in metabolism when switching between the two different hosts. These adaptations involve also substantial changes in the proteome of the glycosome. Comparative (non-quantitative) analysis of BSF and PCF glycosomes by nano LC-ESI-Q-TOF-MS resulted in the validation of known functional aspects of glycosomes and the identification of novel glycosomal constituents.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Glycolysis / physiology*
  • Host-Parasite Interactions / physiology
  • Mass Spectrometry
  • Microbodies / physiology*
  • Proteomics*
  • Protozoan Proteins / blood
  • Protozoan Proteins / metabolism*
  • Trypanosoma brucei brucei / physiology*

Substances

  • Protozoan Proteins