Isolation of Drosophila genes encoding G protein-coupled receptor kinases

Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11067-70. doi: 10.1073/pnas.88.24.11067.


G protein-coupled receptors are regulated via phosphorylation by a variety of protein kinases. Recently, termination of the active state of two such receptors, the beta-adrenergic receptor and rhodopsin, has been shown to be mediated by agonist- or light-dependent phosphorylation of the receptor by members of a family of protein-serine/threonine kinases (here referred to as G protein-coupled receptor kinases). We now report the isolation of a family of genes encoding a set of Drosophila protein kinases that appear to code for G protein-coupled receptor kinases. These proteins share a high degree of sequence homology with the bovine beta-adrenergic receptor kinase. The presence of a conserved family of G protein-coupled receptor kinases in vertebrates and invertebrates points to the central role of these kinases in signal transduction cascades.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Western
  • Cyclic AMP-Dependent Protein Kinases*
  • DNA / genetics
  • DNA / isolation & purification
  • DNA Probes
  • Drosophila / enzymology
  • Drosophila / genetics*
  • Drosophila Proteins
  • Eye Proteins*
  • G-Protein-Coupled Receptor Kinase 1
  • G-Protein-Coupled Receptor Kinase 2
  • GTP-Binding Proteins / analysis
  • GTP-Binding Proteins / genetics*
  • Molecular Sequence Data
  • Multigene Family*
  • Polymerase Chain Reaction / methods
  • Protein Kinases / analysis
  • Protein Kinases / genetics*
  • Receptors, Cell Surface / analysis
  • Receptors, Cell Surface / genetics*
  • Sequence Homology, Nucleic Acid
  • beta-Adrenergic Receptor Kinases


  • DNA Probes
  • Drosophila Proteins
  • Eye Proteins
  • Receptors, Cell Surface
  • DNA
  • Protein Kinases
  • Cyclic AMP-Dependent Protein Kinases
  • G-Protein-Coupled Receptor Kinase 1
  • beta-Adrenergic Receptor Kinases
  • G-Protein-Coupled Receptor Kinase 2
  • Gprk2 protein, Drosophila
  • GTP-Binding Proteins

Associated data

  • GENBANK/M80493
  • GENBANK/M80494
  • GENBANK/M80723
  • GENBANK/M80724
  • GENBANK/M81151
  • GENBANK/M81664
  • GENBANK/M81665
  • GENBANK/M81666
  • GENBANK/S74124
  • GENBANK/S74131