Mapping novel traits by array-assisted bulk segregant analysis in Saccharomyces cerevisiae

Genetics. 2006 Jul;173(3):1813-6. doi: 10.1534/genetics.106.057927. Epub 2006 Apr 19.


We demonstrate a new method, microarray-assisted bulk segregant analysis, for mapping traits in yeast by genotyping pooled segregants. We apply a probabilistic model to the progeny of a single cross and as little as two microarray hybridizations to reliably map an auxotrophic marker, a Mendelian trait, and a major-effect quantitative trait locus.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Chromosome Mapping / methods*
  • Chromosome Segregation
  • Genotype
  • Oligonucleotide Array Sequence Analysis / methods*
  • Probability
  • Quantitative Trait Loci*
  • Saccharomyces cerevisiae / genetics*