To assess the frequency of hyperprolactinemia and evaluate its possible clinical significance in patients with systemic lupus erythematosus (SLE). We determined serum prolactin (PRL) levels in 30 patients with SLE by a radioimmunometric assay. For each patient, the clinical disease activity was assessed using the Systemic Lupus Activity Measure. Antinuclear antibodies were determined by standard techniques. Correlation between PRL concentrations and SLE clinical and serological activity were evaluated. Elevated serum concentrations of PRL (>25 ng/ml in female and >16 ng/ml in male) were found in 10 of the 30 (33.3%) patients (7-85 ng/ml, mean 33.8, SD 19.8). A significant correlation was found between the PRL levels and the clinical disease activity of SLE (P < 0.001, r = 0.675). In addition, hyperprolactinemia was associated with serological activity. Hyperprolactinemia was frequently detected in patients with SLE. There is a significant correlation between hyperprolactinemia and lupus activity.