Rapid Bidirectional Allele-Specific PCR Identification for Triazine Resistance in Higher Plants

Pest Manag Sci. 2006 Jun;62(6):531-6. doi: 10.1002/ps.1197.

Abstract

Triazine resistance is reported to be due to chloroplast herbicide target insensitivity in most species, and this is most often caused by a Ser(264)-Gly mutation at the D1 protein. In order to ascertain whether this mutation is really predominant amongst resistant plants, and also for gene flow studies, a rapid test is needed that allows the testing of large quantities of plants. Here a bidirectional allele-specific PCR (polymerase chain reaction) identification is proposed. The designed primers were shown to be universal in the three grass and three broadleaf species examined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Base Sequence
  • DNA Mutational Analysis
  • DNA Primers
  • Drug Resistance / genetics*
  • Electrophoresis, Agar Gel / methods
  • Herbicides / pharmacology*
  • Molecular Sequence Data
  • Photosystem II Protein Complex / genetics*
  • Poaceae
  • Polymerase Chain Reaction / methods*
  • Triazines / pharmacology*

Substances

  • DNA Primers
  • Herbicides
  • Photosystem II Protein Complex
  • Triazines