Potential contribution of NF-kappaB in neuronal cell death in the glutathione peroxidase-1 knockout mouse in response to ischemia-reperfusion injury

Stroke. 2006 Jun;37(6):1533-8. doi: 10.1161/01.STR.0000221708.17159.64. Epub 2006 Apr 20.

Abstract

Background and purpose: We have previously identified an increased susceptibility of Gpx1(-/-) mice to increased infarct size after middle cerebral artery occlusion (MCAO). This study was designed to elucidate the mechanisms involved in elevated neuronal cell death arising from an altered endogenous oxidant state.

Methods: Gpx1(-/-) mice were exposed to transient MCAO and reperfusion by intraluminal suture blockade. Protein expression of the p65 subunit of transcription factor nuclear factor-kappaB (NF-kappaB) was examined by immunohistochemistry and Western Analysis. NF-kappaB DNA-protein activity was assessed by electrophoretic mobility shift assays (EMSA). Wild-type and Gpx1(-/-) mice were exposed to MCAO with or without the NF-kappaB inhibitor, pyrrolidinedithiocarbamate (PDTC).

Results: Upregulation of the p65 subunit of NF-kappaB and subsequent p65 phosphorylation at serine 536 was detected in the Gpx1(-/-) brains after stroke. EMSAs revealed that increased ischemia-enhanced DNA binding of NF-kappaB was observed in Gpx1(-/-) mice compared with wild-type. Supershift assays indicated that the p50 and p65 subunits participated in the bound NF-kappaB complex. The NF-kappaB inhibitor PDTC, a potential antioxidant, was able to afford partial neuroprotection in the Gpx1(-/-) mice.

Conclusions: NF-kappaB is upregulated in the Gpx1(-/-) mouse, and this upregulation contributes to the increased cell death seen in the Gpx1(-/-) after MCAO. The activation of NF-kappaB may increase the expression of downstream target genes that are involved in the progression of neural injury after MCAO.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Brain Ischemia / physiopathology*
  • Cell Death
  • Cerebral Infarction / pathology
  • DNA / metabolism
  • Glutathione Peroxidase / deficiency*
  • Mice
  • Mice, Knockout
  • NF-kappa B / metabolism*
  • NF-kappa B p50 Subunit / metabolism
  • Neurons / metabolism*
  • Neuroprotective Agents / pharmacology
  • Phosphorylation
  • Pyrrolidines / pharmacology
  • Reperfusion Injury / physiopathology*
  • Stroke / metabolism
  • Thiocarbamates / pharmacology
  • Tissue Distribution
  • Transcription Factor RelA / metabolism
  • Up-Regulation

Substances

  • Antioxidants
  • NF-kappa B
  • NF-kappa B p50 Subunit
  • Neuroprotective Agents
  • Pyrrolidines
  • Thiocarbamates
  • Transcription Factor RelA
  • pyrrolidine dithiocarbamic acid
  • DNA
  • glutathione peroxidase GPX1
  • Glutathione Peroxidase