Stromal cell derived factor-1 acutely promotes neural progenitor cell proliferation in vitro by a mechanism involving the ERK1/2 and PI-3K signal pathways

Cell Biol Int. 2006 May;30(5):466-71. doi: 10.1016/j.cellbi.2006.01.007. Epub 2006 Mar 6.


Stromal cell derived factor-1 (SDF-1), a member of the chemotactic cytokine family, has attracted attention in recent years. It participates in diverse processes such as the regulation of neuronal migration and activation of CD4+ T cells; it is also a co-receptor for human immunodeficiency virus-1 (HIV-1). Here, we show that the proliferation of neural progenitor cells dissociated from rat cortex and cultured in vitro with basic fibroblast growth factor (bFGF) is stimulated by SDF-1. PD98059 and wortmannin, which are, respectively, specific inhibitors of the extracellular regulated kinase1/2 (ERK1/2) and phosphatidylinositol-3 kinase (PI-3K) signal pathways, markedly attenuate this stimulation of proliferation. These findings indicate that SDF-1 acutely promotes the proliferation of NPCs in vitro involving the ERK1/2 and PI-3 kinase pathways, suggesting that it plays a basic role in the development of neural progenitors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Proliferation / drug effects*
  • Cells, Cultured
  • Chemokine CXCL12
  • Chemokines, CXC / pharmacology*
  • Mesenchymal Stem Cells / drug effects*
  • Mitogen-Activated Protein Kinase 1 / metabolism*
  • Mitogen-Activated Protein Kinase 3 / metabolism*
  • Nervous System / embryology
  • Phosphatidylinositol 3-Kinases / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, CXCR4 / metabolism
  • Signal Transduction / drug effects


  • CXCL12 protein, human
  • Chemokine CXCL12
  • Chemokines, CXC
  • Cxcr4 protein, rat
  • Receptors, CXCR4
  • Phosphatidylinositol 3-Kinases
  • Mitogen-Activated Protein Kinase 1
  • Mitogen-Activated Protein Kinase 3