Hydrogen peroxide-induced cell and tissue injury: protective effects of Mn2+

Inflammation. 1991 Aug;15(4):291-301. doi: 10.1007/BF00917314.


Recent evidence indicates that under in vitro conditions, superoxide anion and hydrogen peroxide (H2O2) are unstable in the presence of manganese ion (Mn2+). The current studies show that in the presence of Mn2+, H2O2-mediated injury of endothelial cells is greatly attenuated. A source of bicarbonate ion and amino acid is required for Mn2+ to exert its protective effects. Injury by phorbol ester-activated neutrophils is also attenuated under the same conditions. EDTA reverses the protective effects. Acute lung injury produced in vivo in rats by intratracheal instillation of glucose-glucose oxidase is almost completely blocked in rats treated with Mn2+ and glycine. Conversely, treatment of rats with EDTA, a chelator of Mn2+, markedly accentuates lung injury caused by glucose-glucose oxidase. These data are consistent with the findings of others that Mn2+ can facilitate direct oxidation of amino acids with concomitant H2O2 disproportionation. This could form the basis of a new therapeutic approach against oxygen radical-mediated tissue injury.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Antioxidants / therapeutic use
  • Bicarbonates / pharmacology
  • Capillary Permeability / drug effects
  • Cells, Cultured
  • Cytotoxicity, Immunologic
  • Edetic Acid / pharmacology
  • Endothelium, Vascular / cytology
  • Free Radicals
  • Glucose / toxicity
  • Glucose Oxidase / toxicity
  • Glycine / pharmacology
  • Humans
  • Hydrogen Peroxide / antagonists & inhibitors
  • Hydrogen Peroxide / toxicity*
  • Lung Diseases / chemically induced
  • Lung Diseases / metabolism
  • Lung Diseases / prevention & control*
  • Manganese / pharmacology*
  • Manganese / therapeutic use
  • Neutrophils / immunology
  • Oxidation-Reduction
  • Oxygen / metabolism
  • Pulmonary Artery / cytology
  • Rats
  • Superoxides / metabolism


  • Antioxidants
  • Bicarbonates
  • Free Radicals
  • Superoxides
  • Manganese
  • Edetic Acid
  • Hydrogen Peroxide
  • Glucose Oxidase
  • Glucose
  • Oxygen
  • Glycine