Identification and functional characterization of levS, a gene encoding for a levansucrase from Leuconostoc mesenteroides NRRL B-512 F

Gene. 2006 Jul 5;376(1):59-67. doi: 10.1016/j.gene.2006.02.007. Epub 2006 Apr 24.


A Leuconostoc mesenteroides NRRL B-512 F levansucrase gene, (levS), was isolated, sequenced and cloned in Escherichia coli. The recombinant enzyme was shown to be a fructosyltransferase producing a polymer identified by (13)C-NMR as levan. Based on sequence analysis, we found that this levansucrase is a mosaic protein, bearing structural features of glucosyltransferases in the amino and carboxy terminal regions similarly to inulosucrase from Leuconostoc citreum. The phylogenetic analysis of the C-terminal region domain of levansucrases from L. mesenteroides demonstrates that they group together into a novel putative sub-family of genes and evolved long before all other glucosyltransferases, while their catalytic domain structure is species related.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Escherichia coli
  • Gene Expression
  • Hexosyltransferases / biosynthesis
  • Hexosyltransferases / genetics*
  • Leuconostoc / enzymology
  • Leuconostoc / genetics*
  • Molecular Sequence Data
  • Phylogeny*
  • Protein Structure, Tertiary
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics
  • Sequence Homology, Amino Acid


  • Bacterial Proteins
  • Recombinant Proteins
  • Hexosyltransferases
  • levansucrase