Identification of a dopamine- and 3'5'-cyclic adenosine monophosphate-regulated phosphoprotein of 32 kD (DARPP-32) in parathyroid hormone-producing cells of the human parathyroid gland

J Endocrinol Invest. 1991 Sep;14(8):655-61. doi: 10.1007/BF03347888.


The cellular localization of DARPP-32, a dopamine- and cyclic AMP-regulated phosphoprotein of 32 kD, enriched in dopamine-innervated brain regions, was investigated in the human parathyroid gland using indirect immunofluorescence histochemistry. Monoclonal antibodies were used to demonstrate DARPP-32-like immunoreactivity (LI) in chief cells of the normal human parathyroid gland and in cells of human parathyroid adenoma. Direct double-labelling revealed coexistence of DARPP-32-LI with parathyroid hormone (PTH)-LI. It has previously been demonstrated that dopamine D1-receptors are present in the parathyroid gland and that dopamine and D1-agonists stimulate the release of PTH. The present results suggest that DARPP-32 may play a role in the cellular mechanisms leading to dopamine-induced PTH secretion.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoma / chemistry
  • Cyclic AMP / physiology*
  • Dopamine / physiology*
  • Dopamine and cAMP-Regulated Phosphoprotein 32
  • Fluorescent Antibody Technique
  • Humans
  • Molecular Weight
  • Nerve Tissue Proteins / isolation & purification*
  • Parathyroid Glands / cytology
  • Parathyroid Glands / metabolism*
  • Parathyroid Hormone / biosynthesis*
  • Parathyroid Neoplasms / chemistry
  • Phosphoproteins / isolation & purification*


  • Dopamine and cAMP-Regulated Phosphoprotein 32
  • Nerve Tissue Proteins
  • Parathyroid Hormone
  • Phosphoproteins
  • Cyclic AMP
  • Dopamine