Polymorphism of the PE domain of PE/PE_PGRS sequences in clinical isolates of Mycobacterium bovis in Mexico

Vet Microbiol. 2006 Jul 20;115(4):364-9. doi: 10.1016/j.vetmic.2006.02.021. Epub 2006 Apr 25.

Abstract

Polymorphism of the PE domain of PE/PE_PGRS sequences was studied in Mycobacterium bovis isolates from different Mexican states. Samples were analyzed by spolygotyping and RFLP using IS6110 and a 235-bp fragment of the PE domain of PE/PE_PGRS as probes. With the PE probe, three different genotypes were observed, one being predominant in all states. These results confirm the high conservation of the PE domain and suggests a potential role for PE sequence as a stable genetic marker for bovine tuberculosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Typing Techniques / veterinary
  • Base Sequence
  • Cattle
  • DNA Primers
  • DNA, Bacterial / analysis*
  • Genetic Markers
  • Genotype
  • Mexico
  • Mycobacterium bovis / classification*
  • Mycobacterium bovis / genetics*
  • Phylogeny*
  • Polymorphism, Restriction Fragment Length*
  • Tuberculosis, Bovine / diagnosis

Substances

  • DNA Primers
  • DNA, Bacterial
  • Genetic Markers