Genome-wide analysis of high-altitude maize and gene knockdown stocks implicates chromatin remodeling proteins in response to UV-B

Plant J. 2006 May;46(4):613-27. doi: 10.1111/j.1365-313X.2006.02721.x.

Abstract

A comparative analysis, by expression profiling of maize, was performed to identify novel components in the mechanisms of maize responses to UV-B. Five high-altitude landraces grown from 2,000 to 3,400 m naturally receive higher UV-B fluence than plants at lower altitudes and similar latitudes. These high-altitude landraces were compared directly with a low-altitude line and with literature reports for other temperate maize lines. A microarray analysis demonstrated that among the UV-B responsive transcripts, several types of gene implicated in chromatin remodeling are differentially expressed before and after UV-B treatment in high-altitude lines. RNAi transgenic plants with lower expression of four such chromatin-associated genes exhibited hypersensitivity to UV-B by measurements of leaf arching, increased leaf chlorosis and necrosis, and altered UV-B regulation of selected genes. These results collectively suggest that genes involved in chromatin remodeling are crucial for UV-B acclimation and that some high-altitude lines exhibit adaptations to this challenge.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adaptation, Physiological
  • Chromatin Assembly and Disassembly / genetics*
  • Cluster Analysis
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Nuclear Proteins / physiology
  • Oligonucleotide Array Sequence Analysis
  • Plant Leaves / anatomy & histology
  • Plant Leaves / metabolism
  • Plant Leaves / radiation effects
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plant Proteins / physiology
  • Plants, Genetically Modified / metabolism
  • Plants, Genetically Modified / radiation effects
  • RNA Interference
  • RNA, Messenger / metabolism
  • Ultraviolet Rays*
  • Zea mays / genetics*
  • Zea mays / metabolism
  • Zea mays / radiation effects*

Substances

  • Nuclear Proteins
  • Plant Proteins
  • RNA, Messenger