A multiplex PCR for beta-lactamase genes of Haemophilus influenzae and description of a new blaTEM promoter variant

J Antimicrob Chemother. 2006 Jul;58(1):183-5. doi: 10.1093/jac/dkl150. Epub 2006 Apr 26.

Abstract

Objectives: To establish a multiplex PCR to detect and differentiate the beta-lactamase genes of Haemophilus influenzae.

Methods: A collection of H. influenzae isolates with a range of different beta-lactamase genes were tested in parallel with individual PCRs and the multiplex assay developed in this study.

Results: The multiplex method was found to be superior to previous methods and was able to correctly detect and differentiate blaROB-1 and the variants of blaTEM present in the strains tested, including a previously unrecognized variant associated with a 54 bp insertion in the promoter region.

Conclusions: The multiplex PCR will be a useful tool for the surveillance of beta-lactamase genes in H. influenzae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Drug Resistance, Multiple, Bacterial
  • Genetic Variation
  • Haemophilus influenzae / enzymology*
  • Polymerase Chain Reaction / methods*
  • Promoter Regions, Genetic / genetics*
  • beta-Lactam Resistance*
  • beta-Lactamases / genetics*
  • beta-Lactams / pharmacology

Substances

  • Anti-Bacterial Agents
  • beta-Lactams
  • beta-Lactamases