Identification of chicken embryo kinase 5, a developmentally regulated receptor-type tyrosine kinase of the Eph family

Cell Regul. 1991 Jul;2(7):523-34. doi: 10.1091/mbc.2.7.523.


Chicken embryo kinase 5 (Cek5) is a transmembrane tyrosine kinase of the Eph family that was identified by screening a 10-d chicken embryo cDNA expression library with anti-phosphotyrosine antibodies. The extracellular region of Cek5 contains a cysteine rich N-terminal subdomain and a C-terminal subdomain mostly devoid of cysteines and comprising two repeats similar to fibronectin type III repeats. Immunoblotting experiments with anti-Cek5 polyclonal antibodies indicated that Cek5 is a membrane-associated 120-kDa protein containing intramolecular (but not intermolecular) disulfide bonds. Cek5 is already expressed in 2-d-old chicken embryos and is also expressed, at higher levels, later in development. In 10-d-old chicken embryos, Cek5 is expressed at substantial levels in nearly all the tissues examined, whereas in adult it is expressed predominantly in the brain. The expression of Cek5 in the brain gradually diminishes during embryonic development, whereas in the skeletal muscle of the thigh a sharp decrease in Cek5 expression was detected at the time of terminal muscle differentiation. Its wide tissue distribution throughout development and its sustained expression in adult brain suggest that Cek5 is an important component of signal transduction pathways, likely to interact with a widely distributed and important ligand, which is as yet unknown.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chick Embryo
  • Gene Expression Regulation, Enzymologic / physiology*
  • Histocompatibility Antigens Class II
  • Molecular Sequence Data
  • Open Reading Frames
  • Protein-Tyrosine Kinases / analysis*
  • Protein-Tyrosine Kinases / chemistry
  • Protein-Tyrosine Kinases / genetics
  • Protein-Tyrosine Kinases / physiology
  • Receptor, EphB2
  • Receptors, Cell Surface / chemistry*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / physiology
  • Sequence Alignment
  • Signal Transduction / physiology


  • Histocompatibility Antigens Class II
  • Ia-like antigens, avian
  • Receptors, Cell Surface
  • Protein-Tyrosine Kinases
  • Receptor, EphB2

Associated data

  • GENBANK/M62325
  • GENBANK/S60911
  • GENBANK/S60914
  • GENBANK/S60917
  • GENBANK/S60919
  • GENBANK/S60920
  • GENBANK/S60969
  • GENBANK/S61524
  • GENBANK/S67999
  • GENBANK/S81999