Evaluation of a novel strip test, GenoType Mycobacterium CM/AS, for species identification of mycobacterial cultures

Clin Microbiol Infect. 2006 May;12(5):481-3. doi: 10.1111/j.1469-0691.2006.01380.x.

Abstract

A novel DNA strip assay, GenoType Mycobacterium AS, was evaluated for its ability to identify 219 mycobacterial isolates in combination with the GenoType Mycobacterium CM assay. The results were compared with those obtained by conventional 16S rDNA sequencing. The Genotype test correlated well (96%) with sequencing. However, with the CM kit alone, it was possible to identify most (88%) of the isolates found in clinical specimens, and the AS kit provided very little additional information.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Humans
  • Mycobacterium / classification*
  • Mycobacterium / genetics
  • Mycobacterium / isolation & purification
  • Mycobacterium Infections / microbiology*
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 23S / chemistry
  • RNA, Ribosomal, 23S / genetics
  • Reagent Kits, Diagnostic*
  • Sensitivity and Specificity

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 23S
  • Reagent Kits, Diagnostic