YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex

FEBS Lett. 2006 May 15;580(11):2553-60. doi: 10.1016/j.febslet.2006.04.002. Epub 2006 Apr 12.

Abstract

In Saccharomyces cerevisiae the pyruvate dehydrogenase (PDH) complex is regulated by reversible phosphorylation of its Pda1p subunit. We here provide evidence that Pda1p is phosphorylated by the mitochondrial kinase Yil042cp. Deletion of YOR090c, encoding a putative mitochondrial phosphatase, results in a decreased PDH activity, indicating that Yor090cp acts as the corresponding PDH phosphatase. We demonstrate by means of blue native gel electrophoresis and tandem affinity purification that both enzymes are associated with the PDH complex.

MeSH terms

  • Amino Acid Sequence
  • Conserved Sequence
  • Humans
  • Mitochondria / metabolism
  • Mitochondrial Proteins / metabolism
  • Molecular Sequence Data
  • Phosphorylation
  • Protein Binding
  • Protein Kinases / chemistry
  • Protein Kinases / metabolism*
  • Protein Subunits / genetics
  • Protein Subunits / metabolism
  • Protein-Serine-Threonine Kinases
  • Pyruvate Dehydrogenase (Acetyl-Transferring) Kinase
  • Pyruvate Dehydrogenase (Lipoamide)-Phosphatase / chemistry
  • Pyruvate Dehydrogenase (Lipoamide)-Phosphatase / metabolism*
  • Pyruvate Dehydrogenase Complex / chemistry
  • Pyruvate Dehydrogenase Complex / genetics
  • Pyruvate Dehydrogenase Complex / metabolism*
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • Mitochondrial Proteins
  • Protein Subunits
  • Pyruvate Dehydrogenase (Acetyl-Transferring) Kinase
  • Pyruvate Dehydrogenase Complex
  • Saccharomyces cerevisiae Proteins
  • Protein Kinases
  • Protein-Serine-Threonine Kinases
  • Pyruvate Dehydrogenase (Lipoamide)-Phosphatase