Preventive effect of oral administration of 6-(methylsulfinyl)hexyl isothiocyanate derived from wasabi (Wasabia japonica Matsum) against pulmonary metastasis of B16-BL6 mouse melanoma cells

Cancer Detect Prev. 2006;30(2):174-9. doi: 10.1016/j.cdp.2005.10.005. Epub 2006 Apr 27.


Aim: Effect of oral administration of 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC) or a 6-MITC-containing T-wasabi fraction from wasabi root (Wasabia japonica Matsum) to inhibit the macroscopic pulmonary metastasis was studied with a murine B16-BL6 melanoma model.

Method: Two administration routes, subcutaneous or intravenous, and two administration times, prior to or concomitant with tumor inoculation, of 6-MITC or T-wasabi against the metastatic foci formation in C57BL/6J mouse lungs were compared.

Results: The number of metastasized foci per lung in either subcutaneous or intravenous injection was significantly reduced by intake of 6-MITC or a T-wasabi fraction. The maximum reduction by a T-wasabi fraction reached to 82%. Fifty-six percent of foci formation was inhibited by a 2 week-prior administration of 6-MITC (200 microM), whereas only 27% inhibition was obtained by a concomitant administration with tumor inoculation. Neither 6-MITC nor T-wasabi at tested concentrations showed any toxic effects.

Discussion: Together with our previous results, a component of the Japanese pungent spice, wasabi appears to inhibit not only tumor cell growth but also tumor metastasis. Therefore, 6-MITC from wasabi is apparently a useful dietary candidate for controlling tumor progression.

MeSH terms

  • Administration, Oral
  • Animals
  • Antineoplastic Agents / administration & dosage
  • Antineoplastic Agents / pharmacology*
  • Cell Line, Tumor / drug effects
  • Cell Proliferation / drug effects
  • Isothiocyanates / administration & dosage
  • Isothiocyanates / chemical synthesis
  • Isothiocyanates / pharmacology*
  • Lung Neoplasms / drug therapy*
  • Lung Neoplasms / prevention & control
  • Lung Neoplasms / secondary*
  • Male
  • Melanoma, Experimental / drug therapy*
  • Melanoma, Experimental / prevention & control
  • Melanoma, Experimental / secondary*
  • Mice
  • Mice, Inbred C57BL
  • Phytotherapy*
  • Plant Preparations / pharmacology
  • Plant Roots
  • Wasabia*
  • Xenograft Model Antitumor Assays


  • 6-(Methylsulfinyl)hexyl isothiocyanate
  • Antineoplastic Agents
  • Isothiocyanates
  • Plant Preparations