Lesions in the central nervous system of DBA/2 mice infected with the D variant of encephalomyocarditis virus (EMC-D)

J Vet Med Sci. 1991 Dec;53(6):1013-7. doi: 10.1292/jvms.53.1013.


The central nervous system (CNS) of DBA/2 mice inoculated i.p. with the D variant of encephalomyocarditis virus (EMC-D) (10(3) PFU/head) was examined up to 28 days postinoculation (28 DPI). The virus titer of CNS reached a maximum level at 4 DPI, and infectious viruses became undetectable by 28 DPI. Histopathologically, degeneration of neurons with virus antigens was observed in St. pyramidale hippocampi, Nuc. amigdaloideus corticalis and St. granulosum cerebelli of the brain and in Cornu ventrale of the thoracic to lumbar spinal cord at 6 DPI. In addition, in the spinal cord, demyelination was found in Funiculus ventralis and Funiculus lateralis at 6 DPI and it progressed to form spongiosis at 10 DPI. In correspondence with these virological and histopathological findings, hind limb paralysis developed in some mice at 6 DPI, and its incidence increased markedly to about 60% at 10 DPI.

MeSH terms

  • Animals
  • Brain / microbiology
  • Brain / pathology
  • Central Nervous System / microbiology*
  • Central Nervous System / pathology
  • Encephalomyocarditis virus / growth & development
  • Encephalomyocarditis virus / physiology*
  • Enterovirus Infections / microbiology
  • Enterovirus Infections / pathology
  • Enterovirus Infections / veterinary*
  • Hindlimb
  • Male
  • Mice
  • Mice, Inbred DBA*
  • Paralysis / microbiology
  • Paralysis / pathology
  • Paralysis / veterinary
  • Rodent Diseases / microbiology*
  • Rodent Diseases / pathology
  • Spinal Cord / microbiology
  • Spinal Cord / pathology
  • Viral Plaque Assay