Identification of a new cancer/germline gene, KK-LC-1, encoding an antigen recognized by autologous CTL induced on human lung adenocarcinoma

Cancer Res. 2006 May 1;66(9):4922-8. doi: 10.1158/0008-5472.CAN-05-3840.


The purpose of our present study is to identify a tumor-specific antigen capable of inducing a specific cellular immune response in lung cancer patients. We established a lung adenocarcinoma cell line, designated as F1121L, and induced tumor-specific CTL clone H1 from regional lymph node lymphocytes of patient F1121. CTL clone H1 lysed autologous tumor cells in an HLA-B*1507-restricted manner, but not autologous EBV-B, phytohemagglutinin-blast cells, and K562. The CTL clone also recognized allogeneic HLA-B*1501- or 1507-positive lung cancer cell lines in the HLA-restricted manner. Using the CTL clone, we identified an antigen-coding gene by cDNA expression cloning technique. The gene consisted of 556 bp, including an open reading frame consisted of 113 amino acids, designated as Kita-kyushu lung cancer antigen 1 (KK-LC-1). A 9-mer peptide (KK-LC-1(76-84); RQKRILVNL) was identified as an epitope peptide. The genomic DNA of this antigen was located in chromosome Xq22. A reverse transcription-PCR analysis revealed that the mRNA of this gene was only expressed in the testis among normal tissues. It was expressed in 9 of 18 (50%) allogeneic non-small-cell lung cancer cell lines and in 40 of 100 (40%) non-small-cell lung cancer tissues. We thus identified a new tumor antigen-coding gene categorized as a cancer/germline gene by an autologous lung cancer and CTL system. The new cancer/germline gene was located in Xq22, which is apparently different from the locations of previously reported cancer/germline genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics*
  • Adenocarcinoma / immunology*
  • Aged
  • Antigens, Neoplasm / genetics*
  • Antigens, Neoplasm / immunology
  • Cell Line, Tumor
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA, Complementary / immunology
  • Epitopes / genetics
  • Epitopes / immunology
  • HLA-A Antigens / immunology
  • HLA-B Antigens / immunology
  • Humans
  • K562 Cells
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*


  • Antigens, Neoplasm
  • DNA, Complementary
  • Epitopes
  • HLA-A Antigens
  • HLA-B Antigens