Retinal [125I]iodomelatonin Binding Sites in the Tree Shrew (Tupaiidae)

Neurosci Lett. 1991 Sep 16;130(2):149-52. doi: 10.1016/0304-3940(91)90384-6.

Abstract

The characteristics of melatonin-binding sites labelled by [125I]iodomelatonin in membrane preparations from the tree shrew retina were determined. Specific binding of [125I]iodomelatonin to the membrane preparations of tree shrew retina was rapid, stable, saturable, and reversible. Among the indoles tested only 6-chloromelatonin, melatonin and N-acetylserotonin had significant affinities to the [125I]iodomelatonin binding site. Scatchard analysis of the membrane preparations revealed a dissociation constant (Kd) of 51.0 +/- 16 pM and a total number of binding sites (Bmax) of 1.97 +/- 0.6 fmol/mg protein.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding, Competitive
  • Indoles / metabolism
  • Kinetics
  • Melatonin / metabolism*
  • Membrane Proteins / metabolism
  • Receptors, Melatonin
  • Receptors, Neurotransmitter / analysis*
  • Receptors, Neurotransmitter / metabolism
  • Retina / chemistry*
  • Structure-Activity Relationship
  • Tupaiidae / anatomy & histology*
  • Tupaiidae / metabolism

Substances

  • Indoles
  • Membrane Proteins
  • Receptors, Melatonin
  • Receptors, Neurotransmitter
  • Melatonin