Single-nucleotide-polymorphism-specific PCR for quantification and discrimination of Chlamydia pneumoniae genotypes by use of a "locked" nucleic acid

Jan Rupp; Werner Solbach; Jens Gieffers

doi:10.1128/AEM.72.5.3785-3787.2006

Single-nucleotide-polymorphism-specific PCR for quantification and discrimination of Chlamydia pneumoniae genotypes by use of a "locked" nucleic acid

Appl Environ Microbiol. 2006 May;72(5):3785-7. doi: 10.1128/AEM.72.5.3785-3787.2006.

Authors

Jan Rupp¹, Werner Solbach, Jens Gieffers

Affiliation

¹ Institute of Medical Microbiology and Hygiene, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany.

Abstract

Single-nucleotide polymorphisms (SNPs) are targets to discriminate intraspecies diversity of bacteria and to correlate a genotype with a potential pathotype. Quantification of polygenotypic populations supports this task for in vitro and in vivo applications. We present a novel assay capable of quantifying mixtures of two genotypes differing by only one SNP.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques
Chlamydophila pneumoniae / classification*
Chlamydophila pneumoniae / genetics*
Chlamydophila pneumoniae / isolation & purification
DNA Primers
Genotype
HeLa Cells
Humans
Oligonucleotides
Oligonucleotides, Antisense / genetics*
Polymerase Chain Reaction / methods*
Polymorphism, Single Nucleotide*

Substances

DNA Primers
Oligonucleotides
Oligonucleotides, Antisense
locked nucleic acid