Transformation of maize via Agrobacterium tumefaciens using a binary co-integrate vector system

Methods Mol Biol. 2006;318:315-23. doi: 10.1385/1-59259-959-1:315.

Abstract

This chapter describes a stepwise protocol to achieve success in genetic transformation of maize using Agrobacterium tumefaciens as a DNA delivery system. Researchers will be able to effectively transform immature embryos of Hi-II and related genotypes with this protocol. The outcome of the transformation process will be transgenic embryogenic callus tissue, transgenic plants, and transgenic progeny seeds. Recommendations for molecular confirmation and evaluation of transgenic tissue/plants are also provided.

MeSH terms

  • Agrobacterium tumefaciens / genetics*
  • Agrobacterium tumefaciens / isolation & purification
  • Agrobacterium tumefaciens / physiology
  • Gene Transfer Techniques*
  • Genetic Vectors / genetics
  • Plants, Genetically Modified
  • Regeneration
  • Seeds
  • Transformation, Genetic / genetics*
  • Zea mays / genetics*
  • Zea mays / microbiology
  • Zea mays / physiology