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. 1991 Dec;39(12):3368-9.
doi: 10.1248/cpb.39.3368.

Low Molecular Weight Heparin Enhances Prostacyclin Production by Cultured Human Endothelial Cells

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Low Molecular Weight Heparin Enhances Prostacyclin Production by Cultured Human Endothelial Cells

T Kaji et al. Chem Pharm Bull (Tokyo). .

Abstract

Confluent cultures of vascular endothelial cells derived from the human umbilical vein were incubated in a serum-free medium in the presence of low molecular weight heparin (LMWH) with molecular weights of 4000-6000 dalton (Da), or of unfractionated heparin (UFH) with average molecular weight 12,000 Da, and prostacyclin production was determined by radioimmunoassay for 6-keto-prostaglandin F1 alpha, the stable metabolite of prostacyclin. LMWH at 1 U/ml as anti-factor Xa activity significantly increased prostacyclin production after 6h or longer; however, UFH at 1 USP U/ml did not induce such a significant change. The LMWH-induced increase in prostacyclin production occurred at 0.1 U/ml and above after 6 h of treatment. Since prostacyclin is both a potent inhibitor of platelet aggregation and a vasodilator, it was suggested that the increased endothelial cell prostacyclin production induced by LMWH may be a component of the anticoagulant activity of the drug.

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