Cerato-platanin, the first member of a new fungal protein family: cloning, expression, and characterization

Cell Biochem Biophys. 2006;44(3):512-21. doi: 10.1385/CBB:44:3:512.

Abstract

The ascomycete Ceratocystis fimbriata, the causal agent of "canker stain disease," secretes a protein of 12.4 kDa that elicits phytoalexin synthesis and plant cell death. This protein, named cerato-platanin (CP), is also located in the cell walls of ascospores, hyphae, and conidia; it contains four cysteines (S-S bridged) and is moderately hydrophobic. The cp gene consists of a single exon and has 42 bp codifying for a signal peptide of 14 residues. The recombinant protein was obtained by cloning the cp gene of the mature protein in Escherichia coli (BL21), and a refolding step was needed to achieve the native active form. In the European Molecular Biology data bank, CP is reported as the first member of the CP family; this is the first example of an set of secreted fungal proteins whose primary structure is very similar. Nonetheless, the data also revealed some structural and functional features that make CP similar to proteins of the hydrophobin family.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence / genetics
  • Chromosome Mapping / methods
  • Cloning, Molecular / methods
  • Escherichia coli / genetics
  • Fungal Proteins / classification
  • Fungal Proteins / genetics*
  • Fungal Proteins / isolation & purification*
  • Fungal Proteins / metabolism
  • Gene Expression / genetics*
  • Genes, Fungal
  • Hyphae / metabolism
  • Molecular Sequence Data
  • Phytoalexins
  • Plant Extracts / metabolism
  • Protein Folding
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Sequence Alignment
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Sesquiterpenes
  • Spores, Fungal / metabolism
  • Terpenes

Substances

  • Cerato-platanin protein, Ceratocystis fimbriata
  • Fungal Proteins
  • Plant Extracts
  • Recombinant Proteins
  • Sesquiterpenes
  • Terpenes
  • Phytoalexins