Intrasplenic steady-state dendritic cell precursors that are distinct from monocytes

Nat Immunol. 2006 Jun;7(6):663-71. doi: 10.1038/ni1340. Epub 2006 May 7.


Immediate precursors of the many subtypes of dendritic cells (DCs) remain obscure. Here we purified a splenic precursor population that produced all splenic CD8+ and CD8- conventional DCs (cDCs) but not plasmacytoid DCs or other lineages. This 'pre-cDC' population included cells 'precommitted' to form either CD8+ or CD8- cDCs. The pre-cDCs, which comprised 0.05% of splenocytes, expressed a CD11c(int) CD45RA(lo) CD43(int) SIRP-alpha(int) CD4- CD8- major histocompatibility complex class II-negative surface phenotype. The pre-cDCs were not monocytes. Monocytes generated few cDCs in steady-state recipient mice. However, when transferred into mice with an inflammatory milieu dependent on granulocyte-macrophage colony-stimulating factor, monocytes produced a distinct type of splenic DC. Thus, the inflammatory status of the host influences the developmental origin and type of DC present in lymphoid tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / immunology
  • Biological Assay
  • CD8-Positive T-Lymphocytes / cytology
  • Cell Differentiation*
  • Cell Lineage*
  • Dendritic Cells / cytology*
  • Dendritic Cells / immunology
  • Ferrosoferric Oxide / chemistry
  • Flow Cytometry
  • Mice
  • Mice, Inbred Strains
  • Microspheres
  • Monocytes / cytology
  • Monocytes / immunology
  • Spleen / cytology*
  • Stem Cells / cytology*
  • Stem Cells / immunology


  • Antibodies, Monoclonal
  • Ferrosoferric Oxide