Co-infection of human parvovirus B19 in Vietnamese patients with hepatitis B virus infection

J Hepatol. 2006 Sep;45(3):361-9. doi: 10.1016/j.jhep.2006.03.013. Epub 2006 Apr 27.


Background/aims: Human parvovirus B19 (B19) has been identified in the serum of hepatitis B virus (HBV) infected patients. However, the effect of B19-infection on the course of HBV-associated liver disease has not previously been investigated. We examined the prevalence of B19-DNA in HBV-infected Vietnamese patients and analysed the association between co-infection and the clinical outcome of HBV-infection.

Methods: Serum samples from 399 HBV-infected patients and 64 healthy individuals were analysed for the presence of B19-DNA by PCR and DNA-sequencing.

Results: B19-DNA was detected in 99/463 (21.4%) individuals. The proportion of HBV-infected patients who were also co-infected with B19 was higher than the healthy controls (P<0.001). B19-DNA was detected more frequently in patients with HBV-associated hepatocellular carcinoma compared to patients with acute and chronic HBV, HBV-associated liver cirrhosis and healthy subjects (P<0.006). A positive correlation was also found between B19-DNA loads and both serum HBV-DNA loads and alanine aminotransferase (rho>0.250 and P<0.05).

Conclusions: Our findings demonstrate that B19-infection is frequent in HBV-infected Vietnamese patients. Also, a significant correlation exists between HBV/B19 co-infection and a greater likelihood of progression to more severe hepatitis B-associated liver disease. Further studies are required to determine the role of B19-infection on HBV-associated pathogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Alanine Transaminase / blood
  • Antibodies, Viral / blood
  • Comorbidity
  • DNA, Viral / blood
  • DNA, Viral / genetics
  • Disease Progression
  • Female
  • Genome / genetics
  • Hepatitis B / complications*
  • Hepatitis B / epidemiology*
  • Hepatitis B / immunology
  • Hepatitis B / pathology
  • Hepatitis B virus / genetics
  • Hepatitis B virus / immunology
  • Humans
  • Liver Cirrhosis / pathology
  • Male
  • Middle Aged
  • Parvoviridae Infections / complications*
  • Parvoviridae Infections / epidemiology*
  • Parvoviridae Infections / immunology
  • Parvoviridae Infections / pathology
  • Parvovirus B19, Human / genetics
  • Parvovirus B19, Human / immunology
  • Prevalence
  • Vietnam / epidemiology
  • Viral Load


  • Antibodies, Viral
  • DNA, Viral
  • Alanine Transaminase