Abstract
RNA is not cleaved as a consequence of the binding of RNase H to the duplex between RNA and a complementary alpha-oligodeoxyribonucleotide (oligo). In consequence targets have been selected which do not a priori require the action of RNase H to inhibit genetic expression. Two models have been used: The Friend Murine Leukemia Virus (F-MuLV) and the synthesis of rabbit beta globin.alpha-oligos trigger specific inhibitions in both systems. The functionalisation in 5' with the intercalating agent 9-NH2-ellipticine renders the oligos resistant to degradation and allows a direct action on cells.
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Ellipticines / pharmacology*
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Friend murine leukemia virus / drug effects
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Friend murine leukemia virus / genetics
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Gene Expression / drug effects
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Globins / genetics
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Intercalating Agents / pharmacology*
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Molecular Sequence Data
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Nucleic Acid Conformation
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Oligodeoxyribonucleotides / chemistry
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Oligodeoxyribonucleotides / metabolism
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Oligodeoxyribonucleotides / pharmacology*
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Oligonucleotides, Antisense / metabolism
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Oligonucleotides, Antisense / pharmacology*
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Protein Biosynthesis / drug effects
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RNA, Messenger / drug effects*
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RNA, Viral / drug effects*
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Rabbits
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Ribonuclease H / metabolism*
Substances
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Ellipticines
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Intercalating Agents
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Oligodeoxyribonucleotides
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Oligonucleotides, Antisense
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RNA, Messenger
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RNA, Viral
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9-aminoellipticine
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Globins
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Ribonuclease H