Generation of interleukin-17 receptor-like protein (IL-17RL) in prostate by alternative splicing of RNA

Prostate. 2006 Sep 1;66(12):1268-74. doi: 10.1002/pros.20422.

Abstract

Background: Interleukin-17 receptor-like protein (IL-17RL) expressed in prostate tissues changes with advanced cancers due to extensive alternative splicing, which affects the final protein. Predominant IL-17RL splice isoform variants have not been identified, hindering functional studies.

Methods: A cDNA library of IL-17RL transcripts was arrayed onto nylon membranes. Individual transcript exon structures were determined by successively probing membranes with exon-specific oligonucleotides. The most common variants were transiently over-expressed in 293T cells.

Results: We detected >90 different IL-17RL isoforms. Three most abundant isoforms account for approximately half the total transcripts; the full-length variant just over 11%. Surprisingly, most alternative splicing does not alter the reading frame of the full-length molecule; therefore, resulting proteins vary mostly in N-terminal domains.

Conclusions: IL-17RL exists as multiple isoforms due to extensive alternative splicing. We identified the most abundant splices in prostate tissue and established a technique to investigate changes in RNA IL-17RL splicing that occur in advanced cancers.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alternative Splicing / genetics*
  • Cell Line, Tumor
  • DNA, Neoplasm / genetics
  • Disease Progression
  • Exons / genetics
  • Gene Expression Regulation, Neoplastic / genetics
  • Humans
  • Male
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / metabolism
  • Protein Isoforms / genetics
  • RNA, Neoplasm / genetics*
  • RNA, Neoplasm / metabolism
  • Receptors, Interleukin / genetics*
  • Receptors, Interleukin / metabolism
  • Transcription, Genetic / genetics

Substances

  • DNA, Neoplasm
  • IL17RC protein, human
  • Protein Isoforms
  • RNA, Neoplasm
  • Receptors, Interleukin