Tumor Stem Cells Derived From Glioblastomas Cultured in bFGF and EGF More Closely Mirror the Phenotype and Genotype of Primary Tumors Than Do Serum-Cultured Cell Lines

Cancer Cell. 2006 May;9(5):391-403. doi: 10.1016/j.ccr.2006.03.030.

Abstract

The concept of tumor stem cells (TSCs) provides a new paradigm for understanding tumor biology, although it remains unclear whether TSCs will prove to be a more robust model than traditional cancer cell lines. We demonstrate marked phenotypic and genotypic differences between primary human tumor-derived TSCs and their matched glioma cell lines. Unlike the matched, traditionally grown tumor cell lines, TSCs derived directly from primary glioblastomas harbor extensive similarities to normal neural stem cells and recapitulate the genotype, gene expression patterns, and in vivo biology of human glioblastomas. These findings suggest that TSCs may be a more reliable model than many commonly utilized cancer cell lines for understanding the biology of primary human tumors.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cluster Analysis
  • Epidermal Growth Factor / pharmacology*
  • Fibroblast Growth Factor 2 / pharmacology*
  • Gene Expression Profiling
  • Genome, Human / genetics
  • Genotype
  • Glioblastoma / pathology*
  • Humans
  • Loss of Heterozygosity
  • Mice
  • Mice, SCID
  • Models, Biological
  • Neoplastic Stem Cells / drug effects*
  • Neoplastic Stem Cells / pathology*
  • Phenotype
  • Serum
  • Transcription, Genetic
  • Tumor Cells, Cultured

Substances

  • Fibroblast Growth Factor 2
  • Epidermal Growth Factor

Associated data

  • GEO/GSE4536