POSH, a scaffold protein for JNK signaling, binds to ALG-2 and ALIX in Drosophila

FEBS Lett. 2006 May 29;580(13):3296-300. doi: 10.1016/j.febslet.2006.05.005. Epub 2006 May 8.

Abstract

Plenty of SH3s (POSH) functions as a scaffold protein for the Jun N-terminal kinase (JNK) signal transduction pathway, which leads to cell death in mammalian cultured cells and Drosophila. Here, we show that POSH forms a complex with Apoptosis-linked gene-2 (ALG-2) and ALG-2-interacting protein (ALIX/AIP1) in a calcium-dependent manner. Overexpression of ALG-2 or ALIX in developing imaginal eye discs resulted in roughened or melanized eyes, respectively. These phenotypes were enhanced by co-overexpression of POSH. We found that overexpression of either gene could induce ectopic JNK activation, suggesting that POSH/ALG-2/ALIX may function together in the regulation of the JNK pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium-Binding Proteins / genetics
  • Calcium-Binding Proteins / metabolism*
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cytoskeletal Proteins / genetics
  • Cytoskeletal Proteins / metabolism*
  • Drosophila / chemistry
  • Drosophila / growth & development
  • Drosophila / metabolism*
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Eye / anatomy & histology
  • Eye / growth & development
  • Eye / metabolism
  • Gene Expression Regulation, Developmental
  • Immunoprecipitation
  • MAP Kinase Kinase 4 / metabolism
  • Microfilament Proteins / genetics
  • Microfilament Proteins / metabolism*
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Protein Interaction Mapping
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism

Substances

  • ALIX protein, Drosophila
  • Calcium-Binding Proteins
  • Carrier Proteins
  • Cytoskeletal Proteins
  • Drosophila Proteins
  • Microfilament Proteins
  • Nerve Tissue Proteins
  • POSH protein, Drosophila
  • RNA, Messenger
  • MAP Kinase Kinase 4