Protective effect of Rehmannia glutinosa on the cisplatin-induced damage of HEI-OC1 auditory cells through scavenging free radicals

J Ethnopharmacol. 2006 Oct 11;107(3):383-8. doi: 10.1016/j.jep.2006.03.024. Epub 2006 Apr 6.


The steamed root of Rehmannia glutinosa has been used in traditional Oriental Medicine for treatment of inner ear diseases, such as tinnitus and hearing loss. In the present study, we showed that the ethanol extract of steamed roots of Rehmannia glutinosa (SRG) protected HEI-OC1 auditory cells from cisplatin cytotoxicity in a dose-dependent fashion. In addition, to investigate the protection mechanism of SRG on cisplatin cytotoxicity towards HEI-OC1, we measured the effects of SRG on lipid peroxidation of cisplatin treated cells as well as scavenging activities against superoxide radical, hydroxyl radical, hydrogen peroxide, and DPPH radical. SRG (5-100 microg/ml) had protective effect against the cisplatin-induced HEI-OC1 cell damage and reduced lipid peroxidation in a dose-dependent manner. Furthermore, SRG showed strong scavenging activity against superoxide radical, hydroxyl radical, hydrogen peroxide, and DPPH radical. These results indicate that SRG protects cisplatin-induced HEI-OC1 cell damage through inhibition of lipid peroxidation and scavenging activities of free radials.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / toxicity*
  • Biphenyl Compounds
  • Cell Line
  • Cell Survival / drug effects
  • Cisplatin / toxicity*
  • Cochlea / cytology
  • Cochlea / drug effects*
  • Cochlea / metabolism
  • Dose-Response Relationship, Drug
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / pharmacology*
  • Hydrogen Peroxide / chemistry
  • Hydroxyl Radical / chemistry
  • Lipid Peroxidation / drug effects
  • Picrates / chemistry
  • Plant Extracts / pharmacology
  • Plant Roots
  • Rehmannia*
  • Superoxides / chemistry


  • Antineoplastic Agents
  • Biphenyl Compounds
  • Free Radical Scavengers
  • Picrates
  • Plant Extracts
  • Superoxides
  • Hydroxyl Radical
  • Hydrogen Peroxide
  • 1,1-diphenyl-2-picrylhydrazyl
  • Cisplatin