Measurement of aromatisation by a urine technique suitable for the evaluation of aromatase inhibitors in vivo

J Enzyme Inhib. 1991;4(4):315-25. doi: 10.3109/14756369109030396.


By modification of a recently developed method for separation of radio-labelled urinary oestrogens we were able to separate oestrogen metabolites and measure their isotope ratios in urine following injections of [3H]delta 4-androstenedione and [14C]oestrone. This method provides a useful tool for studying in vivo aromatisation of delta 4-androstenedione into oestrone in breast cancer patients before and during treatment with aromatase inhibitors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoglutethimide / therapeutic use
  • Androstenedione / metabolism
  • Aromatase / metabolism*
  • Aromatase Inhibitors*
  • Breast Neoplasms / drug therapy
  • Breast Neoplasms / metabolism*
  • Estrogens / urine*
  • Estrone / biosynthesis
  • Female
  • Humans


  • Aromatase Inhibitors
  • Estrogens
  • Aminoglutethimide
  • Estrone
  • Androstenedione
  • Aromatase