Light-driven DNA repair by photolyases

Cell Mol Life Sci. 2006 Jun;63(11):1266-77. doi: 10.1007/s00018-005-5447-y.


DNA photolyases are highly efficient light-driven DNA repair enzymes which revert the genome-damaging effects caused by ultraviolet (UV) radiation. These enzymes occur in almost all living organisms exposed to sunlight, the only exception being placental mammals like humans and mice. Their catalytic mechanism employs the light-driven injection of an electron onto the DNA lesion to trigger the cleavage of cyclobutane- pyrimidine dimers or 6-4 photoproducts inside duplex DNA. Spectroscopic and structural analysis has recently yielded a concise view of how photolyases recognize these DNA lesions involving two neighboring bases, catalyze the repair reaction within a nanosecond and still achieve quantum efficiencies of close to one. Apart from these mechanistic aspects, the potential of DNA photolyases for the generation of highly UV-resistant organisms, or for skin cancer prevention by ectopical application is increasingly recognized.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • DNA / radiation effects
  • DNA Repair / genetics*
  • Deoxyribodipyrimidine Photo-Lyase / genetics
  • Deoxyribodipyrimidine Photo-Lyase / physiology*
  • Forecasting
  • Humans
  • Light
  • Models, Biological
  • Models, Molecular
  • Pyrimidine Dimers / metabolism
  • Skin Neoplasms / enzymology
  • Skin Neoplasms / prevention & control
  • Ultraviolet Rays


  • Pyrimidine Dimers
  • pyrimidine-pyrimidone dimer
  • DNA
  • Deoxyribodipyrimidine Photo-Lyase