Monocyte chemotactic protein-1 (MCP-1) acts as a paracrine and autocrine factor for prostate cancer growth and invasion

Prostate. 2006 Sep 1;66(12):1311-8. doi: 10.1002/pros.20464.


Background: Monocyte chemotactic protein-1 (MCP-1) plays a key role in the recruitment and activation of monocytes during inflammation. Increased MCP-1 serum levels in patients with various cancers were correlated with advanced stage. Here, we evaluated the role of MCP-1 on prostate cancer (CaP) cell proliferation and invasion.

Methods: Expression of MCP-1 in tissue specimens was analyzed by immunohistochemical staining. MCP-1 production was determined by ELISA in conditioned media collected from primary prostate epithelia (PrEC), LNCaP, C4-2B, PC3 cells, and hFOB. Cell proliferation and invasion were assayed by MTS assay and invasion chambers.

Results: All CaP cells, as well as hFOB, produced high amount of MCP-1 compared to PrEC cells. MCP-1 expression levels were associated with advanced pathologic stage. MCP-1 induced proliferation and invasion of CaP cells and this was abolished partially either by CCR2 antagonist or PI3 Kinase inhibitor.

Conclusion: MCP-1 acts as a paracrine and autocrine factor for CaP growth and invasion.

MeSH terms

  • Cell Line
  • Cell Line, Tumor
  • Cell Proliferation* / drug effects
  • Chemokine CCL2 / metabolism
  • Chemokine CCL2 / physiology*
  • Chromones / pharmacology
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism
  • Epithelial Cells / pathology
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Immunohistochemistry
  • Male
  • Morpholines / pharmacology
  • Neoplasm Invasiveness / pathology
  • Neoplasm Invasiveness / physiopathology*
  • Osteoblasts / drug effects
  • Osteoblasts / metabolism
  • Phosphoinositide-3 Kinase Inhibitors
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / physiopathology*
  • Receptors, CCR2
  • Receptors, Chemokine / genetics
  • Receptors, Chemokine / metabolism
  • Signal Transduction / drug effects
  • Signal Transduction / physiology


  • CCL2 protein, human
  • CCR2 protein, human
  • Chemokine CCL2
  • Chromones
  • Morpholines
  • Phosphoinositide-3 Kinase Inhibitors
  • Receptors, CCR2
  • Receptors, Chemokine
  • 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one