Human parechovirus types 1, 2 and 3 infections in Canada

Emerg Infect Dis. 2006 Jun;12(6):969-75. doi: 10.3201/eid1206.051675.


A new reverse transcription-polymerase chain reaction assay was developed for identification of 28 Canadian human parechovirus (HPeV) isolates, including 20 HPeV-1, 3 HPeV-2, and 5 HPeV-3, recovered from 1985 to 2004. All HPeV-1 isolates but 1 were genetically distinct from the Harris reference strain. One HPeV-2 isolate was related to the Williamson strain; the other 2 were related to the Connecticut strain. HPeV-3 isolates clustered together. Seventy-five percent of isolates were recovered during the typical enterovirus season. All patients but 1 were children with a mean age of 14.6 months, 6.3 months, and 0.7 months for HPeV-1, HPeV-2, and HPeV-3 patients, respectively. All HPeV-2- and HPeV-3-infected children were hospitalized with a diagnosis of viremia or sepsis. HPeV-1- infected children had bronchiolitis diagnosed in 50% of the cases, with few cases of pneumonia and enteritis. Two infected patients (1 child with leukemia and a 78-year-old woman) died of septic shock and severe pneumonia, respectively.

MeSH terms

  • Aged
  • Amino Acid Sequence
  • Base Sequence
  • Cytopathogenic Effect, Viral
  • HT29 Cells
  • Humans
  • Infant
  • Infant, Newborn
  • Molecular Sequence Data
  • Parechovirus / classification*
  • Parechovirus / genetics
  • Phylogeny
  • Picornaviridae Infections / epidemiology
  • Picornaviridae Infections / virology*
  • Quebec
  • RNA, Viral / chemistry
  • RNA, Viral / genetics
  • Retrospective Studies
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, DNA


  • RNA, Viral