Maltose and maltodextrin utilization by Bacillus subtilis

J Bacteriol. 2006 Jun;188(11):3911-22. doi: 10.1128/JB.00213-06.


Bacillus subtilis can utilize maltose and maltodextrins that are derived from polysaccharides, like starch or glycogen. In this work, we show that maltose is taken up by a member of the phosphoenolpyruvate-dependent phosphotransferase system and maltodextrins are taken up by a maltodextrin-specific ABC transporter. Uptake of maltose by the phosphoenolpyruvate-dependent phosphotransferase system is mediated by maltose-specific enzyme IICB (MalP; synonym, GlvC), with an apparent K(m) of 5 microM and a V(max) of 91 nmol . min(-1) . (10(10) CFU)(-1). The maltodextrin-specific ABC transporter is composed of the maltodextrin binding protein MdxE (formerly YvdG), with affinities in the low micromolar range for maltodextrins, and the membrane-spanning components MdxF and MdxG (formerly YvdH and YvdI, respectively), as well as the energizing ATPase MsmX. Maltotriose transport occurs with an apparent K(m) of 1.4 microM and a V(max) of 4.7 nmol . min(-1) . (10(10) CFU)(-1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / metabolism*
  • Biological Transport
  • Genotype
  • Kinetics
  • Maltose / metabolism*
  • Operon
  • Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism*
  • Plasmids
  • Polysaccharides / chemistry
  • Polysaccharides / metabolism*
  • Restriction Mapping


  • Polysaccharides
  • Maltose
  • maltodextrin
  • Phosphoenolpyruvate Sugar Phosphotransferase System