Identification and characterization of novel and unknown mouse epididymis-specific genes by complementary DNA microarray technology

Biol Reprod. 2006 Sep;75(3):462-8. doi: 10.1095/biolreprod.105.048058. Epub 2006 May 17.


To examine epididymal function, we attempted to identify highly expressed genes in mouse epididymis using a cDNA microarray containing PCR products amplified from a mouse epididymal cDNA library. We isolated one novel and four known genes-lymphocyte cytosolic protein 1 (Lcp1), complement subcomponents C1r/C1s, Uegf protein, and bone morphogenetic protein and zona pellucida-like domains 1 (Cuzd1), transmembrane epididymal protein 1 (Teddm1), and whey acidic protein 4-disulfide core domain 16 (Wfdc16)-with unknown functions in the epididymis. The novel gene, designated Serpina1f (serine peptidase inhibitor [SERPIN], clade A, member 1f), harbors an open reading frame of 1 233 bp encoding a putative protein of 411 amino acids, including a SERPIN domain. These five genes were predominantly expressed in the epididymis as compared to other organs. In situ hybridization analysis revealed their epididymal region-specific expression patterns. Real-time RT-PCR analysis revealed a significant increase in mRNA expression of these genes around puberty. Castration decreased their expression, except forLcp1. Testosterone (T) restored these reduced expressions, except forTeddm1; however, this restoration was not observed with 17 beta-estradiol (E2). Administration of T and E2 combination recovered the Serpina1f mRNA concentration; this recovery was also observed with T alone. However, the recovery of Cuzd1and Wfdc16mRNA concentrations was inadequate. Neonatal diethylstilbestrol treatment suppressed the Cuzd1, Wfdc16, and Serpina1f mRNA expression in the epididymis of 8-week-old mice; this was not observed with E2. These results suggest that our microarray system can provide a novel insight into the epididymal function on a molecular basis, and the five genes might play important roles in the epididymis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Bone Morphogenetic Proteins / biosynthesis
  • Cytoskeletal Proteins
  • Epididymis / drug effects
  • Epididymis / metabolism*
  • In Situ Hybridization
  • Male
  • Mice
  • Mice, Inbred ICR
  • Microfilament Proteins
  • Molecular Sequence Data
  • Oligonucleotide Array Sequence Analysis*
  • Orchiectomy
  • Phosphoproteins / biosynthesis
  • Phosphoproteins / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Steroids / pharmacology


  • Bone Morphogenetic Proteins
  • Cytoskeletal Proteins
  • Microfilament Proteins
  • Phosphoproteins
  • Steroids
  • Lcp1 protein, mouse

Associated data

  • GENBANK/AB233454