A B-lectin receptor kinase gene conferring rice blast resistance

Plant J. 2006 Jun;46(5):794-804. doi: 10.1111/j.1365-313X.2006.02739.x.

Abstract

Rice blast, caused by the fungal pathogen Magnaporthe grisea, is one of the most devastating diseases in rice worldwide. The dominant resistance gene, Pi-d2 [previously named Pi-d(t)2], present in the rice variety Digu, confers gene-for-gene resistance to the Chinese blast strain, ZB15. Pi-d2 was previously mapped close to the centromere of chromosome 6. In this study, the Pi-d2 gene was isolated by a map-based cloning strategy. Pi-d2 encodes a receptor-like kinase protein with a predicted extracellular domain of a bulb-type mannose specific binding lectin (B-lectin) and an intracellular serine-threonine kinase domain. Pi-d2 is a single-copy gene that is constitutively expressed in the rice variety Digu. Transgenic plants carrying the Pi-d2 transgene confer race-specific resistance to the M. grisea strain, ZB15. The Pi-d2 protein is plasma membrane localized. A single amino acid difference at position 441 of Pi-d2 distinguishes resistant and susceptible alleles of rice blast resistance gene Pi-d2. Because of its novel extracellular domain, Pi-d2 represents a new class of plant resistance genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Chromosome Mapping
  • Gene Dosage
  • Genetic Complementation Test
  • Green Fluorescent Proteins / analysis
  • Immunity, Innate / genetics
  • Magnaporthe / pathogenicity*
  • Molecular Sequence Data
  • Oryza / anatomy & histology
  • Oryza / genetics*
  • Oryza / physiology
  • Plant Diseases / microbiology*
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / physiology*
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases / chemistry
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / physiology*
  • Recombinant Fusion Proteins / analysis
  • Sequence Analysis, DNA

Substances

  • Plant Proteins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Protein-Serine-Threonine Kinases