Antigen-specific accumulation of naïve, memory and effector CD4 T cells during anterior uveitis monitored by intravital microscopy

Cell Immunol. 2006 Jan;239(1):49-60. doi: 10.1016/j.cellimm.2006.03.005. Epub 2006 May 19.


Uveitis is an immune-mediated ocular disease and a leading cause of blindness. We characterized a novel model of uveitis with intravital microscopy. Transfer of ovalbumin-specific T cells from DO11.10 spleen to BALB/c recipients and subsequent challenge with ovalbumin in the anterior chamber of the eye resulted in anterior uveitis. Antigen-specificity was verified by injection of irrelevant antigen and transfer of T cells with a different specificity. Subsets of CD4 T cells, including naive (DO11.10 RAG(-/-)) and in vitro-activated Th2 effector CD4 T cells, infiltrated anterior segment tissues early in the inflammation. Memory-like CD44(high) CD4 T cells from unprimed transgenic mice and in vitro-activated Th1 effector CD4 T cells accumulated to larger numbers than naive or Th2 effector cells at 48 and 72 h. Of these, the alpha(2)-integrin+CD4 unprimed T cells entered the eye more efficiently, and antibody to alpha(2)-integrin markedly inhibited the inflammatory response. Intravital microscopy revealed the early arrival and antigen-specific accumulation of CD4 T cells in inflamed tissue and should be helpful in understanding T cell migration to other organs.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antigens / immunology*
  • CD4-Positive T-Lymphocytes / cytology*
  • CD4-Positive T-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / metabolism
  • Cell Count
  • Female
  • Gene Expression Regulation
  • Hyaluronan Receptors / immunology
  • Immunity, Innate / immunology*
  • Immunologic Memory / immunology*
  • Integrin alpha2 / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Microscopy
  • Ovalbumin / pharmacology
  • Uveitis, Anterior / chemically induced
  • Uveitis, Anterior / immunology*
  • Uveitis, Anterior / metabolism
  • Uveitis, Anterior / pathology*


  • Antigens
  • Hyaluronan Receptors
  • Integrin alpha2
  • Ovalbumin