Abstract
We studied the quorum sensing (QS) system and the related homoserine lactones (HSLs) observing Pseudomonas aeruginosa invasion using the epithelial cell monolayer penetration assay model. Compared to the PAO1 wild-type, the QS mutants, DeltalasI and DeltarhlI, were compromised in their capacity to invade. The decreased invasiveness of DeltarhlI was restored by adding 100 microM exogenous C(4)-HSL. However, the decreased invasiveness of an efflux mutant, DeltamexAB-oprM, was not restored in the presence of exogenous HSLs. The QS system partially plays a role in P. aeruginosa invasion; however, C(4)-HSL and 3-O-C(12)-HSL are not the essential determinants for invasiveness for P. aeruginosa.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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4-Butyrolactone / analogs & derivatives*
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4-Butyrolactone / pharmacology
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Animals
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Outer Membrane Proteins / metabolism*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Dogs
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Ligases / genetics
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Ligases / metabolism
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / metabolism*
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Mice
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Pseudomonas aeruginosa / genetics
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Pseudomonas aeruginosa / metabolism
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Pseudomonas aeruginosa / pathogenicity
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Pseudomonas aeruginosa / physiology*
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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LasI protein, Pseudomonas aeruginosa
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Membrane Transport Proteins
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MexA protein, Pseudomonas aeruginosa
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MexB protein, Pseudomonas aeruginosa
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OprM protein, Pseudomonas aeruginosa
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Transcription Factors
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homoserine lactone
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Ligases
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RHLI protein, Pseudomonas aeruginosa
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4-Butyrolactone